{"id":76764,"date":"2023-05-22T06:02:25","date_gmt":"2023-05-22T13:02:25","guid":{"rendered":"https:\/\/ixcellsbiotech.com\/product\/human-astrocytes-ipsc-derived-psen2-mutant-n141ihet\/"},"modified":"2026-07-14T14:28:51","modified_gmt":"2026-07-14T21:28:51","slug":"human-astrocytes-ipsc-derived-psen2_m141i_het","status":"publish","type":"product","link":"https:\/\/ixcellsbiotech.com\/product\/human-astrocytes-ipsc-derived-psen2_m141i_het\/","title":{"rendered":"Human Astrocytes (iPSC- derived, PSEN2 mutant, N141I,HET)"},"content":{"rendered":"

Product Description<\/strong><\/span><\/span><\/p>\n

Astrocytes are a crucial component of the human central nervous system. Astrocytes’ diverse and heterogeneous character allows them to serve a variety of important activities during brain formation as well as afterwards in neuronal homeostasis and synaptic transmission [1]<\/sup> . Since they engage in the development of synapses, and neurotransmitter recycling, astrocytes are crucial for the construction and plasticity of brain circuits [2]<\/sup>. In addition, astrocytes are immunocompetent cells that participate in neuroinflammation by secreting cytokines and chemokines, which may have protective or detrimental consequences for neuronal survival [3]<\/sup> .<\/p>\n

iXCells Biotechnologies<\/strong> is proud to provide fully differentiated and functional human iPSC-derived human astrocytes that display typical astrocytic morphology and express key markers of e.g., GFAP, ALDH1L1 (Figure 1A,B) when cultured in the Human Astrocyte Maintenance Medium (Cat# MD-0109-100ML)<\/a>. In addition, our iPSC-derived Astrocytes can also be co-cultured with cortical neurons or other cell types for drug screening platforms (Figure 1C-E) and can respond to inflammatory stimuli (Figure 2) and moreover affect neurite length of neurons when previously stimulated collected conditioned media is applied (Figure 3).<\/p>\n

\"iXCells<\/p>\n

Figure 1.<\/strong> Human iPSCs derived astrocytes show expression of characteristic biological markers. (A)<\/strong> Immunostaining of iPSC-derived astrocytes expressing ALDH1L1 (green), CD44 (red), S100b (red), or GFAP (green) at day 28. All cells were counterstained for DAPI (blue). Scale bars, 200 \u00b5m. (B)<\/strong> Quantifications for percentage of astrocytes, positive for the listed markers over DAPI. Results are expressed as means \u00b1 SEM. (C)<\/strong> Cortical neurons were differentiated as a monolayer on a MEA plate and cocultured with different types of astrocytes, and subsequently neuronal activity was measured. (D)<\/strong> Example raster plot showing spikes (black lines) and network bursts (pink lines) in the MEA for neurons cultured with or without astrocytes. (E)<\/strong> At 5 days of neural differentiation, electrical parameters were measured in the MEA and the results are graphed as mean \u00b1 SEM for mean firing rate and number of spikes.<\/p>\n

 <\/p>\n

\"40HU<\/p>\n

Figure 2. (A)<\/strong> Representative blots showing expression of cytokines (4-week differentiation) in primary astrocytes and iPSC-derived astrocytes which were either not activated (PBS) or activated (10 ng\/mL Il-1\u03b2 for 24 h). Proteome Profiler Human Cytokine Array Kit was used for detection and data shown are from 2-minute exposure. (B)<\/strong> Legend for the Human Cytokine Array panel with 36 different antibodies to human cytokines, chemokines, and acute phase proteins (done in duplicates). (C)<\/strong> Secretion levels in IL6 and IL8 in cell supernatants in non-treated cells (PBS) and treated cells (Il-1\u03b2).<\/p>\n

\"40HU<\/p>\n

Figure 3. (A)<\/strong> Astrocytes (Cat# 40HU-008) were cultured for 21 days in astrocyte media and then stimulated. Five hours later, cytokines were washed then neuronal media was added. Twenty-four hours later the conditioned media was collected and stored. Dopaminergic neurons (Cat# 40HU-002) were then defrosted in the respective conditioned media and neurite branching was monitored. (B)<\/strong> Representative blots showing expression of cytokines (3-week differentiation) in iPSC-derived astrocytes which were either not activated (PBS) or activated (10 ng\/mL Il-1\u03b2 for 5 h). Proteome Profiler Human Cytokine Array Kit was used for detection and data shown are from 2-minute exposure. Red rectangle highlights secretion of IL6 and IL8 in cell supernatants in nontreated cells (PBS) and treated cells (Il-1\u03b2). (C)<\/strong> Plot of neurite length per cell-body cluster area of dopaminergic neurons grown in conditioned media (stimulated and non-stimulated) over time. The graphs show mean \u00b1 SEM (significance values: *p < 0.01). The neurite length was monitored in real-time using Essen BioScience IncuCyte\u00ae S3.<\/p>\n

Product Details<\/strong><\/p>\n\n\n\n\n\n\n\n
Tissue<\/strong><\/td>\nHuman iPSC-derived Astrocytes<\/td>\n<\/tr>\n
Package Size<\/strong><\/td>\n1.0 million cells\/vial; 2.0 million cells\/vial;<\/td>\n<\/tr>\n
Shipped<\/strong><\/td>\nCryopreserved<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nLiquid nitrogen<\/td>\n<\/tr>\n
Media<\/strong><\/td>\nHuman Astrocytes Maintenance Medium (Cat# MD-0109-100ML)<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/span><\/strong><\/span><\/p>\n

[1] Khakh B.S., Sofroniew M.V. Diversity of astrocyte functions and phenotypes in neural circuits. Nat. Neurosci. 2015;18:942\u2013952.<\/p>\n

[2] Sofroniew M.V., Vinters H.V. Astrocytes: biology and pathology. Acta Neuropathol. 2010;119:7\u201335..<\/p>\n

[3] Colombo E., Farina C. Astrocytes: key regulators of neuroinflammation. Trends Immunol. 2016;37:608\u2013620.<\/p>\n

Datasheet & Culture Protocol<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Product Description Astrocytes are a crucial component of the human central nervous system. Astrocytes’ diverse and heterogeneous character allows them to serve a variety of important activities during brain formation as well as afterwards in neuronal homeostasis and synaptic transmission [1] . Since they engage in the development of synapses, and neurotransmitter recycling, astrocytes are […]<\/p>\n","protected":false},"featured_media":89863,"template":"","meta":{"_acf_changed":false,"content-type":"","jetpack_publicize_message":"{title}\n\n{excerpt}\n\n{url}","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"_wpas_customize_per_network":false},"product_brand":[],"product_cat":[1835,1647],"product_tag":[],"cell_type":[2420],"cell_system":[2426],"organ":[2439],"disease":[2461],"research_area":[],"mutation":[],"mutation_type":[],"disease_model":[],"parental_line_name":[],"class_list":["post-76764","product","type-product","status-publish","has-post-thumbnail","product_cat-diseases-modeling","product_cat-ipsc-ipsc-derived-cells","cell_type-neurons-and-glial-cells","cell_system-nervous-system","organ-brain-central-nervous-system","disease-alzheimers-disease","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"exclude_global_add_ons":[],"addons":[],"acf":[],"yoast_head":"\nHuman Astrocytes (iPSC- derived, PSEN2 mutant, N141I,HET) - iXCells<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ixcellsbiotech.com\/product\/human-astrocytes-ipsc-derived-psen2_m141i_het\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Human Astrocytes (iPSC- derived, PSEN2 mutant, N141I,HET) - iXCells\" \/>\n<meta property=\"og:description\" content=\"Product Description Astrocytes are a crucial component of the human central nervous system. 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