{"id":74194,"date":"2023-05-22T05:43:13","date_gmt":"2023-05-22T12:43:13","guid":{"rendered":"https:\/\/ixcellsbiotech.com\/product\/rat-embryonic-fibroblasts-ref\/"},"modified":"2026-07-13T11:29:39","modified_gmt":"2026-07-13T18:29:39","slug":"rat-embryonic-fibroblasts-ref","status":"publish","type":"product","link":"https:\/\/ixcellsbiotech.com\/product\/rat-embryonic-fibroblasts-ref\/","title":{"rendered":"Rat Embryonic Fibroblasts (REF)"},"content":{"rendered":"<p><span style=\"font-size: 14px\"><span style=\"color: #ff8c00\"><strong>Product Description<\/strong><\/span><\/span><\/p>\n<p style=\"text-align: justify\">Rat Embryonic Fibroblasts (REF) are used to support the growth of rat and human pluripotent stem cells <sup>[1]<\/sup>. REF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. REFs are isolated from rat embryos and used at early passages <sup>[2]<\/sup>. To serve as feeder cells, REF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells.<\/p>\n<p><strong>iXCells Biotechnologies<\/strong> provides high quality Rat Embryonic Fibroblasts (REF), which are isolated from embryonic day 14 rat embryos and cryopreserved at P0, with &gt;1 million cells in each vial. REFs express fibronectin and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. They can further expand for 5 population doublings in Fibroblast Growth Medium (<a href=\"https:\/\/ixcellsbiotech.com\/product\/fibroblast-growth-medium\/\" target=\"_blank\" rel=\"noopener\">Cat# MD-0011<\/a>) under the condition suggested by iXCells Biotechnologies.<\/p>\n<p><img decoding=\"async\" style=\"display: block;margin-left: auto;margin-right: auto\" src=\"https:\/\/ixcellsbiotech.com\/wp-content\/uploads\/2023\/06\/RatEmbryonicFibroblasts.png\" alt=\"RatEmbryonicFibroblasts\" \/><\/p>\n<p style=\"text-align: center\"><strong>Figure 1. <\/strong>Rat Embryonic Fibroblasts (REF) (phase contrast)<\/p>\n<p><strong style=\"font-size: 14px;line-height: 22.4px;color: #ff8c00\">Product Details<\/strong><\/p>\n<table style=\"line-height: 20.7999992370605px;width: 724px\" border=\"1\" cellspacing=\"1\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td style=\"width: 132px\">Tissue<\/td>\n<td style=\"width: 592px\">Rat Embryonic Fibroblasts (REF)<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 132px\">Package Size<\/td>\n<td style=\"width: 592px\">1.0 x 10<sup>6<\/sup> cells\/vial<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 132px\">Passage Number<\/td>\n<td style=\"width: 592px\">P0<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 132px\">Shipped<\/td>\n<td style=\"width: 592px\">Cryopreserved<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 132px\">Storage<\/td>\n<td style=\"width: 592px\">Liquid nitrogen<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 132px\">Growth Properties<\/td>\n<td style=\"width: 592px\">Adherent<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 132px\">Media<\/td>\n<td style=\"width: 592px\"><a href=\"https:\/\/ixcellsbiotech.com\/product\/fibroblast-growth-medium\/\">Fibroblast Growth Medium (Cat# MD-0011)<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong style=\"font-size: 14px;line-height: 22.4px;color: #ff8c00\">References<\/strong><\/p>\n<p>[1] Bradley, A. (1987). Production and analysis of chimaeras. In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, (ed. E. J. Robertson), pp. 113-151. Oxford: IRL Press.<\/p>\n<p>[2] Ping Li, Chang Tong, Ruty Mehrian-Shai, Li Jia, Nancy Wu, Youzhen Yan, Robert E. Maxson, Eric N. Schulze, Houyan Song, Chih-Lin Hsieh, Martin F. Pera, and Qi-Long Ying. (2008) Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts. <em>Cell<\/em>. 135(7): 1299-1310.<\/p>\n<p><a href=\"https:\/\/ixcellsbiotech.com\/wp-content\/uploads\/2023\/06\/iXCells-Rat_Embryonic_Fibroblasts_REF.pdf\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" src=\"https:\/\/ixcellsbiotech.com\/wp-content\/uploads\/2023\/06\/pdf.jpg\" alt=\"\" width=\"30\" height=\"30\" \/>Download Datasheet<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Product Description Rat Embryonic Fibroblasts (REF) are used to support the growth of rat and human pluripotent stem cells [1]. REF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. REFs are isolated from rat embryos and used at early passages [&hellip;]<\/p>\n","protected":false},"featured_media":90447,"template":"","meta":{"_acf_changed":false,"content-type":"","jetpack_publicize_message":"{title}\n\n{excerpt}\n\n{url}","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"_wpas_customize_per_network":false},"product_brand":[],"product_cat":[1632],"product_tag":[],"cell_type":[2417],"cell_system":[],"organ":[],"disease":[2454],"research_area":[],"mutation":[],"mutation_type":[],"disease_model":[],"parental_line_name":[],"class_list":["post-74194","product","type-product","status-publish","has-post-thumbnail","product_cat-animal-primary-cells","cell_type-fibroblasts","disease-normal-healthy","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"exclude_global_add_ons":[],"addons":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Rat Embryonic Fibroblasts (REF) - iXCells<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ixcellsbiotech.com\/product\/rat-embryonic-fibroblasts-ref\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Rat Embryonic Fibroblasts (REF) - iXCells\" \/>\n<meta property=\"og:description\" content=\"Product Description Rat Embryonic Fibroblasts (REF) are used to support the growth of rat and human pluripotent stem cells [1]. 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