{"id":73642,"date":"2023-05-22T05:33:18","date_gmt":"2023-05-22T12:33:18","guid":{"rendered":"https:\/\/ixcellsbiotech.com\/product\/y27632\/"},"modified":"2026-01-16T12:58:36","modified_gmt":"2026-01-16T20:58:36","slug":"y27632","status":"publish","type":"product","link":"https:\/\/ixcellsbiotech.com\/product\/y27632\/","title":{"rendered":"Y27632"},"content":{"rendered":"

Product Description<\/strong><\/span><\/span><\/p>\n

Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor [1]<\/sup>. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency [2]<\/sup>. This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors [3]<\/sup>. Y27632 in combination with Pifithrin-\u00b5 significantly improves cell recovery after cryopreservation [4]<\/sup>.<\/p>\n

<\/div>\n
\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Catalog No.<\/strong><\/span><\/td>\nMD-0025-2MG<\/span><\/td>\n<\/tr>\n
Quantity<\/strong><\/td>\n2mg (10mM in 624.4\u03bcl DMSO)<\/span><\/td>\n<\/tr>\n
Alternate Name<\/strong><\/td>\n(1R,4r)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride<\/span><\/td>\n<\/tr>\n
CAS Number<\/strong><\/td>\n146986-50-7<\/span><\/td>\n<\/tr>\n
Chemical Formula<\/strong><\/td>\nC<\/span>14<\/sub>H<\/span>23<\/sub>Cl<\/span>2<\/sub>N<\/span>3<\/sub>O<\/span><\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n320.3 g\/mol<\/span><\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n>Greater than 98% by HPLC analysis<\/span><\/td>\n<\/tr>\n
Structure<\/strong><\/td>\n\"Y-27632\"<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nMAINTENANCE AND SELF-RENEWAL<\/p>\n
    \n
  • Enhances survival of human embryonic stem (ES) cells when they are dissociated to single cells by preventing dissociation-induced apoptosis (anoikis), thus increasing their cloning efficiency.<\/li>\n
  • Improves embryoid body formation using forced-aggregation protocols.<\/li>\n
  • Increases the survival of cryopreserved single human ES cells after thawing.<\/li>\n
  • Blocks apoptosis of mouse ES-derived neural precursors after dissociation and transplantation.<\/li>\n<\/ul>\n

    REPROGRAMMING<\/p>\n

      \n
    • Direct lineage reprogramming of fibroblasts to mature neurons, in combination with CHIR99021, RepSox, Forskolin, SP600125, G\u00f66983 and Valproic Acid.<\/li>\n<\/ul>\n

      DIFFERENTIATION<\/p>\n

        \n
      • Improves survival of human ES cell monolayers at the initiation of differentiation protocols<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Product Use<\/strong><\/td>\nY27632 is for research use only<\/span>. It is not approved for human or animal use, or for application in vitro<\/em> diagnostic procedures.<\/span><\/td>\n<\/tr>\n
Storage & stability<\/strong><\/td>\nStore aliquots at -20\u00b0C. Stock solutions are stable for 6 months when stored as directed.<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/strong><\/p>\n

[1] Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000) Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983.<\/span><\/p>\n

[2] Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells.Nat Biotechnol 25: 681-686.<\/p>\n

[3] Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008) Inhibition of the Rho\/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors.J. Neurosci Res. 86: 270-280.<\/p>\n

[4] Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. (2010) Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog. 26: 781-788.<\/p>\n

Download Datasheet<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

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This molecule has also been shown to enhance survival during the […]<\/p>\n","protected":false},"featured_media":109298,"template":"","meta":{"_acf_changed":false,"content-type":"","jetpack_publicize_message":"{title}\n\n{excerpt}\n\n{url}","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"_wpas_customize_per_network":false},"product_brand":[],"product_cat":[1634],"product_tag":[],"cell_type":[],"cell_system":[],"organ":[],"disease":[],"research_area":[],"mutation":[],"mutation_type":[],"disease_model":[],"parental_line_name":[],"class_list":["post-73642","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-media-reagents","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"exclude_global_add_ons":[],"addons":[],"acf":[],"yoast_head":"\nY27632 - iXCells<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ixcellsbiotech.com\/product\/y27632\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Y27632 - iXCells\" \/>\n<meta property=\"og:description\" content=\"Product Description Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor [1]. 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