{"id":73629,"date":"2023-05-22T05:33:16","date_gmt":"2023-05-22T12:33:16","guid":{"rendered":"https:\/\/ixcellsbiotech.com\/product\/mouse-adipose-derived-stem-cells-brown-fat-madsc-bf\/"},"modified":"2026-07-08T15:05:57","modified_gmt":"2026-07-08T22:05:57","slug":"mouse-adipose-derived-stem-cells-brown-fat-madsc-bf","status":"publish","type":"product","link":"https:\/\/ixcellsbiotech.com\/product\/mouse-adipose-derived-stem-cells-brown-fat-madsc-bf\/","title":{"rendered":"Mouse Adipose-Derived Stem Cells-brown fat (MADSC-bf)"},"content":{"rendered":"<p style=\"line-height: 20.8px\"><span style=\"font-size: 16px\"><span style=\"color: #ff8c00\"><strong>Product Description<\/strong><\/span><\/span><\/p>\n<p>Adipose-derived stem cells (ADSC) are multipotent mesenchymal stem cells (MSC) that are capable of differentiating into adipocytes, osteocytes, chondrocytes etc. <em>in vitro<\/em>. ADSC have been applied in studies including stem cell differentiation, regenerative medicine <sup>[1]<\/sup>, cell therapy, tissue engineering and creation of iPS cell lines.<\/p>\n<p><strong>iXCells Biotechnologies<\/strong> provides high quality Mouse Adipose-Derived Stem Cells, also knowns as preadipocytes, which are isolated from C57BL\/6 mouse inguinal white fat tissue\u00a0or <a href=\"https:\/\/ixcellsbiotech.com\/product-category\/animal-primary-cells\/\">interscapular brown fat tissue<\/a>. These cells are cryopreserved at P1, with &gt;0.5 million cells in each vial and can further expand for 3-4 population doublings in Adipose-derived Stem Cell Growth Medium (<a href=\"https:\/\/ixcellsbiotech.com\/product\/adipose-derived-stem-cell-growth-medium\/\">Cat# MD-0003<\/a>) under the condition suggested by iXCells Biotechnologies. In vitro, mADSC can be differentiated into adipocytes and osteoblasts (Figure 1 and 2) using Adipocyte Differentiation Medium (<a href=\"https:\/\/ixcellsbiotech.com\/product\/adipocyte-differentiation-medium\/\">Cat# MD-0005<\/a>) and Osteogenic Differentiation Medium (<a href=\"https:\/\/ixcellsbiotech.com\/product\/osteogenic-differentiation-medium\/\">Cat# MD-0006<\/a>), separately. These mADSC can be further expanded for no more than 3 passages using Adipose-derived Stem Cell Growth Medium (<a href=\"https:\/\/ixcellsbiotech.com\/product\/adipose-derived-stem-cell-growth-medium\/\">Cat# MD-0003<\/a>). These cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center\"><img fetchpriority=\"high\" decoding=\"async\" style=\"display: block;margin-left: auto;margin-right: auto\" src=\"https:\/\/ixcellsbiotech.com\/wp-content\/uploads\/2023\/06\/Mouse-adipocyte-differentiation-white-fat_2.jpg\" alt=\"Adipocyte differentiation\" width=\"800\" height=\"208\" \/><\/p>\n<p><strong>Figure 1.<\/strong> (A)Mouse<b>\u00a0<\/b>ADSCs (phase contrast).\u00a0<b>(B)<\/b>\u00a0Adipocyte induction (Day 10 post adipogenic induction, phase contrast).\u00a0<b>(C)<\/b>\u00a0Adipocyte induction (Day 10\u00a0post adipogenic induction, Oil Red O staining).<\/p>\n<p style=\"text-align: center\"><img decoding=\"async\" style=\"display: block;margin-left: auto;margin-right: auto\" src=\"https:\/\/ixcellsbiotech.com\/wp-content\/uploads\/2023\/06\/Mouse-ADSC-osteogenic-induction-white-fat.jpg\" alt=\"Osteogenic induction\" width=\"600\" height=\"441\" \/><\/p>\n<p><strong>Figure 2.<\/strong>\u00a0 Mouse ADSCs can be differentiated to into osteoblasts using the osteogenic induction protocol (Alizarin Red S staining, Day 21 post osteogenic induction).<\/p>\n<p>&nbsp;<\/p>\n<p style=\"line-height: 20.8px\"><span style=\"font-size: 16px\"><span style=\"color: #ff8c00\"><strong>Product Details<\/strong><\/span><\/span><\/p>\n<table style=\"line-height: 20.8px;width: 600px\" border=\"1\" cellspacing=\"1\" cellpadding=\"1\">\n<tbody>\n<tr>\n<td><strong>Tissue<\/strong><\/td>\n<td>C57BL\/6 mouse interscapular brown fat tissue<\/td>\n<\/tr>\n<tr>\n<td><strong>Package Size<\/strong><\/td>\n<td>0.5 million cells\/vial<\/td>\n<\/tr>\n<tr>\n<td><strong>Passage Number<\/strong><\/td>\n<td>P1<\/td>\n<\/tr>\n<tr>\n<td><strong>Shipped<\/strong><\/td>\n<td>Cryopreserved<\/td>\n<\/tr>\n<tr>\n<td><strong>Storage<\/strong><\/td>\n<td>Liquid nitrogen<\/td>\n<\/tr>\n<tr>\n<td><strong>Growth Properties<\/strong><\/td>\n<td>Adherent<\/td>\n<\/tr>\n<tr>\n<td><strong>Media<\/strong><\/td>\n<td><a style=\"line-height: 20.8px\" href=\"https:\/\/ixcellsbiotech.com\/product\/adipose-derived-stem-cell-growth-medium\/\">Adipose-derived Stem Cell Growth Medium (Cat# MD-0003)<\/a><br \/>\n<a style=\"line-height: 20.8px\" href=\"https:\/\/ixcellsbiotech.com\/product\/adipocyte-differentiation-medium\/\">Adipocyte Differentiation Medium (Cat# MD-0005)<\/a><br \/>\n<a style=\"line-height: 20.8px\" href=\"https:\/\/ixcellsbiotech.com\/product\/osteogenic-differentiation-medium\/\">Osteogenic Differentiation Medium (Cat# MD-0006)<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<div style=\"line-height: 20.8px\"><\/div>\n<div><\/div>\n<div style=\"line-height: 20.8px\">\n<div>\n<div><\/div>\n<\/div>\n<\/div>\n<div style=\"line-height: 20.8px\"><span style=\"font-size: 16px\"><strong style=\"color: #ff8c00;line-height: 18.9091px\">References<\/strong><\/span><\/div>\n<p>&nbsp;<\/p>\n<div style=\"line-height: 20.8px\"><\/div>\n<div style=\"line-height: 20.8px\">[1]\u00a0 Harasymiak-Krzyzanowska I et al. Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cell Mol Biol Lett. 2013; 18(4): 479-493.<\/div>\n<div><\/div>\n<div><\/div>\n<div>\n<div style=\"line-height: 20.8px\"><\/div>\n<div style=\"line-height: 20.8px\"><a href=\"https:\/\/ixcellsbiotech.com\/wp-content\/uploads\/2023\/06\/Mouse_Adipose-Derived_Stem_Cells_mADSC_V2_1.pdf\" target=\"_blank\" rel=\"noopener\">\u00a0Download Datasheet<\/a><\/div>\n<div style=\"line-height: 20.8px\">\n<div><\/div>\n<div><a href=\"https:\/\/ixcellsbiotech.com\/wp-content\/uploads\/2023\/06\/Adipocyte_Differentiation_Protocol_V2.pdf\" target=\"_blank\" rel=\"noopener\">\u00a0Adipocyte Differentiation Protocol<\/a><\/div>\n<div>\n<div><\/div>\n<div><a href=\"https:\/\/ixcellsbiotech.com\/wp-content\/uploads\/2023\/06\/Osteogenic_Differentiation_Protocol_V2.pdf\" target=\"_blank\" rel=\"noopener\">\u00a0Osteogenic\u00a0Differentiation Protocol<\/a><\/div>\n<div><\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Product Description Adipose-derived stem cells (ADSC) are multipotent mesenchymal stem cells (MSC) that are capable of differentiating into adipocytes, osteocytes, chondrocytes etc. in vitro. ADSC have been applied in studies including stem cell differentiation, regenerative medicine [1], cell therapy, tissue engineering and creation of iPS cell lines. iXCells Biotechnologies provides high quality Mouse Adipose-Derived Stem [&hellip;]<\/p>\n","protected":false},"featured_media":108514,"template":"","meta":{"_acf_changed":false,"content-type":"","jetpack_publicize_message":"{title}\n\n{excerpt}\n\n{url}","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"_wpas_customize_per_network":false},"product_brand":[],"product_cat":[1632],"product_tag":[],"cell_type":[2418],"cell_system":[1721],"organ":[2448],"disease":[2454],"research_area":[],"mutation":[],"mutation_type":[],"disease_model":[],"parental_line_name":[],"class_list":["post-73629","product","type-product","status-publish","has-post-thumbnail","product_cat-animal-primary-cells","cell_type-stem-cells","cell_system-adipose-stromal-system","organ-adipose-tissue","disease-normal-healthy","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"exclude_global_add_ons":[],"addons":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Mouse Adipose-Derived Stem Cells-brown fat (MADSC-bf) - iXCells<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ixcellsbiotech.com\/product\/mouse-adipose-derived-stem-cells-brown-fat-madsc-bf\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Mouse Adipose-Derived Stem Cells-brown fat (MADSC-bf) - iXCells\" \/>\n<meta property=\"og:description\" content=\"Product Description Adipose-derived stem cells (ADSC) are multipotent mesenchymal stem cells (MSC) that are capable of differentiating into adipocytes, osteocytes, chondrocytes etc. in vitro. 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