{"id":73551,"date":"2023-05-22T05:33:03","date_gmt":"2023-05-22T12:33:03","guid":{"rendered":"https:\/\/ixcellsbiotech.com\/product\/human-peripheral-blood-cd14-monocytes-positive-selection\/"},"modified":"2026-06-16T14:52:48","modified_gmt":"2026-06-16T21:52:48","slug":"human-peripheral-blood-cd14-monocytes-positive-selection","status":"publish","type":"product","link":"https:\/\/ixcellsbiotech.com\/product\/human-peripheral-blood-cd14-monocytes-positive-selection\/","title":{"rendered":"Human Peripheral Blood CD14+ Monocytes (Positive Selection)"},"content":{"rendered":"

Product Description<\/strong><\/p>\n

Monocytes are innate blood cells that maintain vascular homeostasis and are early responders to pathogens in acute infections [1, 2]<\/sup>. Monocytes constitute 10~30% of peripheral blood mononuclear cells in the human body. They play multiple roles in immune function including replenishing resident macrophages under normal states. And in response to inflammation signals, monocytes can move quickly (approx. 8\u201312 hours) to sites of infection in the tissues and divide\/differentiate into macrophages and dendritic cells to elicit an immune response [3]<\/sup>. CD14 is an important surface marker for monocytes.<\/p>\n

iXCells Biotechnologies<\/strong> offers CD14+ monocytes isolated from normal human peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic selection. > 90% of the cells are CD14+ as showed by flow cytometric analysis (Figure 1 A). And >53% of monocytes are capable to differentiate into mature dendritic cell (Fig 1B, C).<\/p>\n

\"CD14+<\/p>\n

Figure 1<\/strong>. Human Peripheral Blood CD14+ Monocytes (Positive Selection). (A)<\/strong> >90% of purified cells are CD14 positive. (B, C<\/strong>) Flow cytometric analysis of monocytes dendritic cell differentiation, showed >53% of cells are CD83 positive and >77% cells are CD1a positive.<\/p>\n

Product Details<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n
Tissue<\/strong><\/td>\nNormal human peripheral blood<\/td>\n<\/tr>\n
Package Size<\/strong><\/td>\n1.0×107<\/sup> cells\/vial, 4.0×107<\/sup> cells\/vial<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n>90%<\/td>\n<\/tr>\n
Passage Number<\/strong><\/td>\nP0<\/td>\n<\/tr>\n
Shipped<\/strong><\/td>\nCryopreserved<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nLiquid nitrogen<\/td>\n<\/tr>\n
Growth Properties<\/strong><\/td>\nSuspension<\/td>\n<\/tr>\n
Media<\/strong><\/td>\n Blood Cell Culture Medium (Cat# MD-0007)<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

References<\/b><\/p>\n

[1] Martin Guilliams, Alexander Mildner, and Simon Yona. (2018) \u201cDevelopmental and Functional Heterogeneity of Monocytes\u201d. Immunity, 49: 595-613.<\/p>\n

[2] Prakash Babu Narasimhan, Paola Marcovecchio, Anouk A.J. Hamers, and Catherine C. Hedrick. (2019) \u201cNonclassical Monocytes in Health and Disease\u201d. Annual Reviews of Immunology, 37: 439-456.<\/p>\n

[3] Ziegler-Heitbrock, L (2007). “The CD14+ CD16+ Blood Monocytes: their Role in Infection and Inflammation, Review”. J Leukocyte Biology<\/em> 81<\/strong> (3): 584\u201392.<\/p>\n

 <\/p>\n

Download Datasheet<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Product Description Monocytes are innate blood cells that maintain vascular homeostasis and are early responders to pathogens in acute infections [1, 2]. Monocytes constitute 10~30% of peripheral blood mononuclear cells in the human body. They play multiple roles in immune function including replenishing resident macrophages under normal states. And in response to inflammation signals, monocytes […]<\/p>\n","protected":false},"featured_media":88437,"template":"","meta":{"_acf_changed":false,"content-type":"","jetpack_publicize_message":"{title}\n\n{excerpt}\n\n{url}","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"_wpas_customize_per_network":false},"product_brand":[],"product_cat":[1644],"product_tag":[2254,2253],"cell_type":[2419],"cell_system":[2425],"organ":[2449],"disease":[2454],"research_area":[],"mutation":[],"mutation_type":[],"disease_model":[],"parental_line_name":[],"class_list":["post-73551","product","type-product","status-publish","has-post-thumbnail","product_cat-human-primary-cells","product_tag-cd14","product_tag-cd14-monocytes-positive-selection","cell_type-immune-cells","cell_system-immune-and-hematopoietic-system","organ-blood-peripheral-blood-immune-system","disease-normal-healthy","first","instock","taxable","shipping-taxable","purchasable","product-type-variable"],"exclude_global_add_ons":[],"addons":[],"acf":[],"yoast_head":"\nHuman Peripheral Blood CD14+ Monocytes (Positive Selection) - iXCells<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ixcellsbiotech.com\/product\/human-peripheral-blood-cd14-monocytes-positive-selection\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Human Peripheral Blood CD14+ Monocytes (Positive Selection) - iXCells\" \/>\n<meta property=\"og:description\" content=\"Product Description Monocytes are innate blood cells that maintain vascular homeostasis and are early responders to pathogens in acute infections [1, 2]. 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